The goldline darter is currently known from two widely separated localities, the Cahaba River system in central Alabama and the Coosawattee River system in northern Georgia. Threats to the species include: impoundments; sedimentation; point and non-point source pollutions among others. Spawning occurs in early spring, based on our observations and, like most Percina, the larvae are small and pelagic, probably drifting downriver after hatching.
CFI propagated these darters successfully from 1999 to 2002. Spawning occurred all three years starting the beginning of April and lasting six to eight weeks. Spawning sites were usually sandy areas in eddies behind rocks. Larvae were pelagic and took the typical 28 days to develop all juvenile fins and become benthic. Most of the goldline darters produced during this effort have been provided to the U. S. Environmental Protection Agency for toxicity studies. The remaining propagated fish were given to other researchers at other institutions. Kentucky Department of Fish & Wildlife and Virginia Tech Department of Fisheries and Wildlife performed mussel host suitability tests with the fish.